Identification And Molecular Characterization Of Mycobacterium Bovis And Mycobacterium Paratuberculosis In Wild Cats.
By: Zianab Tariq (2010-VA-234)
| Dr. Muhammad Yasin Tipu.
Contributor(s): Prof. Dr. Zafar Iqbal Chaudhary | Prof. Dr. Aftab Ahmed Anjum.
Material type: 
BookPublisher: 2017Description: 49p.Subject(s): PathologyDDC classification: 2845-T Dissertation note: Cases of wildlife diseased with Mycobacterium species are existing in Pakistan and result in high morbidity and mortality. Vaccine is the only preventive measure, but in wildlife the vaccine administration is a strenuous job. In Pakistan vaccination practice is not up to the mark and vaccination schedules are not being followed. Mycobacterial diseases have gain popularity due to their zoonotic effect. Scat samples from Lahore Safari Zoo and Lahore Zoo were collected and properly labelled. Conventional PCR along with Touchdown PCR was done using universal primer sets of M. bovis and M. paratuberculosis. The amplicons were run on agarose gel and the bands were observed under Gel Doc system.
The objective of the study was to detect the currently prevailing Mycobacterium bovis and mycobacterium avium subspecies paratuberculosis in wild cats in Pakistan.
However the results obtained from different kinds of PCR were negative, showing that the wild cat population of Lahore Zoo Safari as well as Lahore Zoo were free from Mycobacterium bovis and mycobacterium avium subspecies paratuberculosis.
| Item type | Current location | Collection | Call number | Status | Date due | Barcode | Item holds |
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 2845-T (Browse shelf) | Available | 2845-T |
Cases of wildlife diseased with Mycobacterium species are existing in Pakistan and result in high morbidity and mortality. Vaccine is the only preventive measure, but in wildlife the vaccine administration is a strenuous job. In Pakistan vaccination practice is not up to the mark and vaccination schedules are not being followed. Mycobacterial diseases have gain popularity due to their zoonotic effect. Scat samples from Lahore Safari Zoo and Lahore Zoo were collected and properly labelled. Conventional PCR along with Touchdown PCR was done using universal primer sets of M. bovis and M. paratuberculosis. The amplicons were run on agarose gel and the bands were observed under Gel Doc system.
The objective of the study was to detect the currently prevailing Mycobacterium bovis and mycobacterium avium subspecies paratuberculosis in wild cats in Pakistan.
However the results obtained from different kinds of PCR were negative, showing that the wild cat population of Lahore Zoo Safari as well as Lahore Zoo were free from Mycobacterium bovis and mycobacterium avium subspecies paratuberculosis.
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